Open Access

Clinical Features of Primary Vitreoretinal Lymphoma: A Single-center Study


1Department of Ophthalmology, Faculty of Medicine andGraduate School of Medicine, Hokkaido University, Sapporo, Japan

2Department of Hematology, Faculty of Medicine and GraduateSchool of Medicine, Hokkaido University, Sapporo, Japan

3Department of Ophthalmology, Health Sciences University of Hokkaido, Sapporo, Japan

Cancer Diagnosis & Prognosis May-June; 1(2): 69-75 DOI: 10.21873/cdp.10010
Received 21 February 2021 | Revised 04 December 2023 | Accepted 02 April 2021
Corresponding author
Kenichi Namba, MD, Ph.D., Department of Ophthalmology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan. Tel: +81 117065944, Fax: +81 117065948


Background/Aim: This study aimed to demonstrate the clinical outcomes of primary vitreoretinal lymphoma (PVRL). Patients and Methods: Seventeen patients with PVRL who had been treated at Hokkaido University Hospital were enrolled in this study. They were diagnosed based on their cytology, interleukin-10/-6 ratio, and immunoglobulin heavy chain (IgH) gene rearrangement. Results: Diagnostic tests detected cytological malignancy among 14 cases (82.3%), high interleukin-10/-6 ratios among 16 cases (94.1%), and IgH monoclonality in 13 cases (76.5%). Systemic corticosteroids were given to seven (41.2%) patients before their diagnosis of PVRL. Treatments after diagnosis comprised intravitreal methotrexate injection, local radiation, and intravenous chemotherapy for 11, seven, and five cases, respectively. Central nervous system and systemic involvements were observed in nine and one case, respectively, and these complications occurred at 3 to 43 months (mean=16 months) after initial ocular presentation. Conclusion: Many of our patients did not receive any systemic intervention, and almost half of patients with PVRL developed central nervous system involvement during their follow-up period.
Keywords: Vitreoretinal lymphoma, diagnosis, treatment, central nervous system involvement

Intraocular lymphoma is a vision-threatening malignant tumor that is likely life-threatening when it has invaded the central nervous system. Malignant lymphomas with intraocular involvement are pathologically classified as either vitreoretinal lymphoma (VRL) or uveal lymphoma. VRL comprises primary VRL (PVRL) and intraocular invasion of primary central nervous system lymphoma (PCNSL). The frequency of VRL has been shown to be low among patients who had recently consulted a uveitis clinic for a specific etiology (1). We recently reported that patients with VRL characteristically manifest vitreal haze, followed by subretinal infiltrates (2). For patients with these ocular findings, a differential diagnosis of VRL predominates, especially if these patients have medical histories of PCNSL or systemic lymphoma. The prognosis of secondary VRL is likely to depend on management of the primary extraocular lesions, and it appears poorer than the prognosis of PVRL (3).

On the contrary, early and accurate diagnoses of PVRL of patients without past medical histories are most challenging compared to those having such history because they have no specific clinical findings corresponding to PVRL. Moreover, according to a multicenter study (4), the rate of PVRL diagnosis via cytological analysis of vitreous specimens was not high, although cytology-based proof of malignant cells in the vitreous fluid is the most critical aspect of a definite diagnosis. We and other researchers have shown that a cell-block preparation using shed vitreous samples improved the rate of cytological PVRL diagnosis (5-7). However, about four-fifths of patients diagnosed with PVRL consequently developed CNS lesions during their lifetime (8). Therefore, importantly, ophthalmologists and uveitis specialists must share their knowledge of the clinical features of PVRL. Accordingly, the present study aimed to report treatment options and clinical outcomes for patients with PVRL at our single center.

Patients and Methods

This study was retrospective and observational. The Institutional Review Board of Hokkaido University approved this study with the use of clinical data (IRB number: 18-90). Informed consent was obtained by the opt-out method on a website explaining the procedures performed and the review of medical records; however, written informed consent was obtained from the representative patient for the case report and accompanying images.

Seventeen patients were diagnosed with PVRL at Hokkaido University Hospital from 2005 to 2016. Clinical features – including fundus examination findings, optical coherence tomography findings, diagnostic rates, ocular/systemic treatments, CNS involvement, and clinical outcomes – were searched for in patients’ medical records. All patients had undergone diagnostic vitrectomy with a 23- or 25-gauge vitreous cutter (2,9). Both undiluted vitreous fluids and diluted shed vitreous fluids were eventually isolated.

Patients were diagnosed with PVRL based on cytological malignancy, an interleukin (IL) 10/IL6 concentration ratio greater than 1, and monoclonal immunoglobulin heavy chain (IgH) gene rearrangement in surgically excised vitreous samples, according to our previous studies (2,5). This study’s exclusion criteria comprised a diagnosis of uveitis, a history of extraocular lymphoma, and the detection of extraocular lymphoma via a systemic survey during the patient’s initial ocular presentation. Any presence of CNS involvement was carefully checked for using brain magnetic resonance imaging (MRI) and positron-emission tomography-computed tomography.

All patients diagnosed with PVRL were referred to our center’s hematologist and neurologists, and their courses of treatment were determined. Most patients underwent only local treatments because of minor side-effects, and some patients required systemic therapy that was, in some cases, combined with local radiotherapy.

Systemic therapies comprised systemic chemotherapy, including intravenous rituximab/methotrexate/procarbazine/vincristine (R-MPV) chemotherapy and high-dose methotrexate injection. Whole-brain radiation therapy was included when patients were young (less than 50 years old) and for selected patients, in conjunction with hematologists and neurologists. Systemic chemotherapy was performed in combination with radiotherapy based on a previous protocol (10).

Local therapies in the current study comprised local external-beam radiation to the affected eye and repeated intravitreal methotrexate injection (IV-MTX). Local radiation was mostly performed up to 2012, and IV-MTX was performed thereafter. IV-MTX was performed once weekly for at least 8 weeks, until vitreous haze and subretinal infiltrates had diminished, at a dose of 400 μg in 0.1 ml in the pars plana using a 30-gauge needle. Patients further underwent IV-MTX maintenance therapy monthly for a year. The outcome of IV-MTX therapy was evaluated, and this evaluation was modified based on a previous report describing intravitreal rituximab administration (11). When the patient’s vitreous haze had diminished and their subretinal infiltrates had become retinal atrophy, they were evaluated as having undergone complete remission.

Comparisons of cases at PVRL diagnosis and during CNS involvement between the study’s clinically symptomatic and asymptomatic groups described below were performed with an unpaired Student’s t-test. A p-value of less than 0.05 was considered statistically significant.


Clinical features and diagnosis. Table I summarizes the clinical features of the patients with PVRL examined in this study. These patients comprised eight men and nine women. Their mean age at PVRL onset was 67 (range=47-87) years. Their mean follow-up duration was 55 (range=6-161) months. Bilateral and unilateral onset were seen for eight and nine patients, respectively, at their initial presentation. Various vitreous haze types were detected across patients, and 10 out of 17 cases involved aurora-type haze (12). Subretinal exudates emerged among six patients, which were also confirmed using optical coherence tomography. Fourteen (82.3%) patients were found to be cytologically positive for malignant cells through conventional smear cytology and cell-block preparations in four (23.5%) and 13 (76.5%) cases, respectively. Furthermore, the cell-block method demonstrated diffuse large B-cell lymphoma in all cases examined with additional immunocytochemistry. IL10/IL6 ratios greater than 1 were noted for 16 (94.1%) patients, with values of 3,141 (range=10-21,100) pg/ml for IL10 and 86 (range=9-567) pg/ml for IL6. IgH monoclonality was detected in 13 (76.5%) cases.

Treatments and clinical outcomes. Systemic corticosteroids were given to 7 (41.2%) patients before their PVRL diagnoses were made. Treatments after diagnosis comprised IV-MTX injection in 11 cases, local ocular radiation in seven cases, whole-brain radiation combined with high-dose IV-MTX in one case, and whole-brain radiation with R-MPV chemotherapy in three cases. Hematologists recommended against systemic chemotherapy for six patients due to old age and systemic complications, such as liver dysfunction and ischemic heart disease. Despite treatment, extraocular involvement with CNSL developed in nine cases, and systemic lymph node dissemination developed in one case. The time to extraocular onset of these conditions after PVRL diagnosis ranged from 3 to 43 months (mean=16.2 months). Three out of nine patients who developed CNS involvement were asymptomatic; the remaining six were symptomatic, with dementia in two cases and with facial deformity associated with one case each of facial nerve palsy, dysarthria, falls, and paralysis. The mean time between initial PVRL diagnosis and the onset of CNSL was 6.3±2.5 and 21.1±17.3 months in asymptomatic (N=3) and symptomatic (N=6) cases, respectively; however, no statistically significant difference was observed between the two groups. In this study, two patients eventually died of CNS relapse, at 78 and 124 months after their initial PVRL diagnoses (Table I).

Report of a representative case. A 48-year-old female (case 10) visited our hospital with a complaint of blurred vision in both eyes in December 2012. Her visual acuities were 1.2 in her right eye (OD) and 0.7 in her left eye (OS). She had anterior chamber inflammation (2+ cells with keratic precipitates) and vitreous haze. Routine blood tests and chest X-rays at our uveitis clinic showed no abnormal findings. Oral prednisolone was given at 30 mg/day, and her visual acuity gradually improved. Prednisolone treatment was tapered and stopped in September 2013. However, this patient was referred to our hospital because vitreous opacity recurred with phlebitis-like findings in December 2013. Her past medical and family history were unremarkable. At the time of the patient’s referral to our hospital, her visual acuities were 0.06 (1.2×−5.25D) OD and 0.06 (1.5×−5.00D) OS, with normal intraocular pressure. A slit-lamp examination revealed that the OD anterior chamber was clear, with a trace flare and occasional cells of the OS. A fundus examination revealed OD subretinal exudates and 1+ vitreous haze of the OS (Figure 1A and B). Pathological survey of the patient’s vitreous fluid obtained during vitrectomy showed cytological malignancy for VRL, monoclonal IgH gene rearrangement, and high IL10/IL6 ratio (IL10: 128 pg/dl, IL6: 25.2 pg/dl). Gadolinium-enhanced MRI detected no brain abnormalities (Figure 2A). The patient was diagnosed with PVRL. She underwent IV-MTX (400 μg) eight times for both eyes. She also received whole-brain radiotherapy targeting the eye globes at a dosage of 23.4 Gy in addition to five courses of R-MPV chemotherapy. Her ocular lesions gradually regressed and then completely disappeared (Figures 1C and D). Although she had undergone routine MRI every 6 months, the patient suddenly complained of dysarthria 43 months after her initial ocular presentation. An MRI revealed massive white-matter lesions, indicating CNSL (Figure 2B). The patient then received high-dose MTX and cytarabine.


In this study, systemic corticosteroids were administered to seven patients before they were definitively diagnosed with PVRL, indicating that idiopathic uveitis was considered as a possibility for some patients. PVRL has been regarded as a masquerading syndrome by ophthalmologists, since accurate diagnosis remains challenging when based on routine clinical examinations. Therefore, suspicious cases in which physicians can barely differentiate between PVRL and uveitis require early vitrectomy for diagnostic sampling, especially when ocular symptoms do not favorably respond to corticosteroid treatments.

Malignant cells were cytologically detected in the vitreous fluids of 14 PVRL cases in this study, together with IL10/IL6 ratios greater than 1 and monoclonal IgH gene rearrangement in 16 and 13 cases, respectively. Previous studies have demonstrated that a positive cytokine profile facilitates an accurate diagnosis of PVRL, with sensitivity and specificity both over 0.8, while the sensitivity and specificity of IgH monoclonality are 0.96 and 1.0, respectively (13,14). In contrast, the positive rate of cytological diagnosis was not favorable, accounting for only about 20-40% among patients with VRL when conventional smear cytology was applied to the undiluted vitreous fluid (5,14). In this study, cytological examinations were conducted with not only conventional smear cytology but also a cell-block preparation, which likely contributed to the study’s relatively high diagnostic rate of VRL compared to others testing only conventional smear cytology (5).

In the present study, nine (52.9%) patients developed CNS involvement, for which the mean time that had elapsed from their initial PVRL diagnosis was 16.2 months. Of patients with PVRL, 65-90% were demonstrated to experience CNSL complications within 2 years of their PVRL diagnosis (8,15), revealing higher rates of CNS involvement compared to our study. One reason for this difference may be this study’s relatively short follow-up periods (less than 2 years in four cases). Another possible reason might be our university hospital’s high cytological diagnostic rate (82.3%) using the cell-block method. Cytological diagnosis is considered the gold standard for definitive VRL diagnosis by ophthalmologists, allowing for early diagnosis and immediate intervention with appropriate treatments. Further studies are needed to clarify the correlation between early PVRL diagnosis and the prevention of future CNSL.

The mean time that elapsed between PVRL diagnosis and CNS involvement in this study (16.2 months) was similar to previous reports (8,15). It was possible to classify our patients with PVRL who suffered a later development of CNS involvement into asymptomatic and symptomatic cases. In asymptomatic cases, CNS involvement was routinely and repeatedly examined with brain MRI and coincidently detected before neurological symptoms appeared. In contrast, symptomatic patients had already presented with various neurological symptoms when CNS involvement was detected. Although no significant difference between these two groups was observed in the time taken to detect CNS involvement, detection in asymptomatic cases tended to be earlier than in symptomatic cases (6.3±2.5 versus 21.1±17.3 months). Routine and repeated brain imaging tests may contribute to the early detection of CNSL in patients with PVRL with no neurological symptoms.

After discussion with hematologists and neurologists, three patients (cases 10, 16, and 17 in Table I) were treated with a combination of whole-brain irradiation and systemic chemotherapy as their initial therapy, based on a previous protocol (10), when no CNS involvement was noted. Notably, these patients remain alive, and one of them is CNSL-free while the other two developed CNSL after 9 and 43 months of follow-up, respectively. Some reports have demonstrated the role of systemic chemotherapy or whole-brain radiotherapy in prolonging life prognoses among patients with PVRL. Cheah et al. retrospectively analyzed 11 patients with PVRL who had undergone R-MPV chemotherapy and local ocular radiation, combined with high-dose cytarabine. Their estimated 4-year overall survival rate was 76%, and three (36%) patients developed CNS involvement during the study’s mean follow-up period of 4.2 years (16). Kaburaki et al. conducted a prospective study using R-MPV and low-dose whole-brain radiotherapy for 11 patients with PVRL. They concluded that their patients’ 4-year overall survival rate was 89%, with CNS involvement occurring for one (9%) patient during the study’s mean follow-up period of 4.1 years. Although white-matter abnormalities detected by brain MRI increased after the study’s combined treatment regimen, only one patient developed mild cognitive impairment (17). Akiyama et al. analyzed a single-arm prospective study using systemic high-dose methotrexate, followed by IV-MTX, for 10 patients with PVRL. Four (40%) of their patients developed CNS involvement during the study’s mean follow-up period of 2.5 years. Seven out of nine (78%) patients treated with IV-MTX alone (one patient was not followed-up) were shown to have survived after their initial PVRL diagnoses (18). These results reflect a fraction of real-world outcomes for patients with PVRL, suggesting that the majority of patients can continue living for several years and avoid severe adverse events caused by systemic chemotherapy and radiation treatment. A multi-center study’s 5-year survival rate was 61% for 217 patients with VRL examined, of whom 83% had PVRL (4). Klimova et al. recently reported that 5-year overall survival rate of patients with PVRL exceeded the corresponding rate for those with PCNSL with and without ocular involvement, highlighting the importance of combination therapy instead of local treatment alone (19). Since whether systemic chemotherapy or radiation treatment should be used for patients with PVRL remains controversial (20), comparative trials are needed alongside real-world outcomes to further improve prognosis of patients with PVRL.

In this study, we have summarized the concept of performing local and systemic PVRL treatments at our center. As a recent expert meeting confirmed the usefulness of IV-MTX in managing patients with VRL (21), we have also recognized IV-MTX as a useful local therapy to temporarily eliminate lymphoma cells in the eye. Both systemic chemotherapy and whole-brain irradiation are responsible for suppressing CNS involvement, but they are performed for younger patients without serious systemic complications and do have severe side-effects, including leukoencephalopathy, which threaten quality of life. Therefore, treatment strategies at our center are as follows: a) IV-MTX is tolerated and used only to preserve visual function, and b) systemic chemotherapy and whole-brain irradiation are performed to prevent CNS involvement, but this indication is limited.

Myeloid differentiation primary response 88 (MYD88) is known to activate nuclear factor-kappa B pathways, the specific mutation of which plays important roles in development of VRL. We recently published data showing no mutation of MYD88 was detected among four patients with VRL, indicating that MYD88 mutation might not frequently occur in patients in Hokkaido, Japan (2). On the other hand, these results differ from other research showing highly frequent MYD88 mutation among patients with VRL (22). Miserocchi et al. recently demonstrated that MYD88 mutation analyses are possible using the aqueous humor, and this finding contributed to differential diagnoses and patient management (23). Therefore, MYD88 is likely a novel diagnostic and therapeutic target applicable worldwide (21), whereas the clinicopathological features of VRL might differ across races or origins. In Japanese populations, 5-year-survival rates have stood at about 60% for patients with VRL (4). Recently, Klimova et al. demonstrated that 5-year survival rates were high for patients with PVRL compared to those with ocular involvement of CNSL or PCNSL without ocular involvement (19). Since controversies persist regarding the use of systemic chemotherapy for patients with PVRL, further comparative studies between prospective clinical trials and real-world outcomes for patients with PVRL are needed.

The current study had some limitations. Firstly, it retrospectively included a small number of patients with PVRL and a relatively short follow-up period (mean=55 months). Secondly, no standardized follow-up method was available in terms of periodical brain MRI evaluation, although this study indicated a trend of shorter duration before CNS involvement in asymptomatic patients compared to symptomatic ones. The present study confirmed that, despite prompt intervention following an early definite diagnosis by our cytological methods, almost half of the patients with PVRL developed extraocular progression during follow-up, especially in the CNS.

Conflicts of Interest

The Authors declare that we have no conflicts of interest.

Authors’ Contributions

SK collected all clinical data and wrote the article. KN, KS, KH, TI, and NK evaluated ophthalmological data and reviewed the article. MO analyzed neurological findings and reviewed the article. SI supervised the data collection and critically revised the article.


1 Ohguro N Sonoda KH Takeuchi M Matsumura M & Mochizuki M The 2009 prospective multi-center epidemiologic survey of uveitis in Japan. Jpn J Ophthalmol. 56(5) 432 - 435 2012. PMID: 22752308. DOI: 10.1007/s10384-012-0158-z
2 Kase S Namba K Kanno-Okada H Onozawa M Hidaka D Iwata D Mizuuchi K Fukuhara T Fukuhara J Kitaichi N Matsuno Y & Ishida S Immunohistochemical and immunocytochemical analyses in patients with vitreoretinal lymphoma. Ocul Immunol Inflamm. 28(1) 147 - 155 2020. PMID: 30335535. DOI: 10.1080/09273948.2018.1533984
3 Karakawa A Taoka K Kaburaki T Tanaka R Shinozaki-Ushiku A Hayashi H Miyagi-Maeshima A Nishimura Y Uekusa T Kojima Y Fukayama M Kurokawa M & Aihara M Clinical features and outcomes of secondary intraocular lymphoma. Br J Haematol. 183(4) 668 - 671 2018. PMID: 29143310. DOI: 10.1111/bjh.15005
4 Kimura K Usui Y Goto H & Japanese Intraocular Lymphoma Study Group Clinical features and diagnostic significance of the intraocular fluid of 217 patients with intraocular lymphoma. Jpn J Ophthalmol. 56(4) 383 - 389 2012. PMID: 22661396. DOI: 10.1007/s10384-012-0150-7
5 Kase S Namba K Iwata D Mizuuchi K Kitaichi N Tagawa Y Okada-Kanno H Matsuno Y & Ishida S Diagnostic efficacy of cell block method for vitreoretinal lymphoma. Diagn Pathol. 11 29 2016. PMID: 26987877. DOI: 10.1186/s13000-016-0479-1
6 Kanno-Okada H Takakuwa E Tagawa Y Kase S Hatanaka KC Hatanaka Y Namba K Mitsuhashi T & Matsuno Y Cytopathologic findings of cell block materials from the vitreous: Diagnostic distinction between intraocular lymphoma and non-lymphomatous diseases. Pathol Int. 67(7) 342 - 349 2017. PMID: 28590047. DOI: 10.1111/pin.12551
7 Raparia K Chang CC & Chévez-Barrios P Intraocular lymphoma: Diagnostic approach and immunophenotypic findings in vitrectomy specimens. Arch Pathol Lab Med. 133(8) 1233 - 1237 2009. PMID: 19653716. DOI: 10.1043/1543-2165-133.8.1233
8 Chan CC Rubenstein JL Coupland SE Davis JL Harbour JW Johnston PB Cassoux N Touitou V Smith JR Batchelor TT & Pulido JS Primary vitreoretinal lymphoma: A report from an international primary central nervous system lymphoma collaborative group symposium. Oncologist. 16(11) 1589 - 1599 2011. PMID: 22045784. DOI: 10.1634/theoncologist.2011-0210
9 Pulido JS Johnston PB Nowakowski GS Castellino A & Raja H The diagnosis and treatment of primary vitreoretinal lymphoma: A review. Int J Retina Vitreous. 4 18 2018. PMID: 29760948. DOI: 10.1186/s40942-018-0120-4
10 Morris PG Correa DD Yahalom J Raizer JJ Schiff D Grant B Grimm S Lai RK Reiner AS Panageas K Karimi S Curry R Shah G Abrey LE DeAngelis LM & Omuro A Rituximab, methotrexate, procarbazine, and vincristine followed by consolidation reduced-dose whole-brain radiotherapy and cytarabine in newly diagnosed primary CNS lymphoma: Final results and long-term outcome. J Clin Oncol. 31(31) 3971 - 3979 2013. PMID: 24101038. DOI: 10.1200/JCO.2013.50.4910
11 Cicinelli MV Marchese A Miserocchi E Giuffré C Berchicci L Querques G Bandello F & Modorati GM Retinal and choroidal changes of vitreoretinal lymphoma from active to remission phase after intravitreal rituximab. Ocul Immunol Inflamm. 28(4) 637 - 646 2020. PMID: 31393199. DOI: 10.1080/09273948.2019.1616769
12 Marchese A Miserocchi E Giuffrè C Cicinelli MV Querques G Bandello F & Modorati G Aurora borealis and string of pearls in vitreoretinal lymphoma: Patterns of vitreous haze. Br J Ophthalmol. 103(11) 1656 - 1659 2019. PMID: 30709808. DOI: 10.1136/bjophthalmol-2018-313491
13 Cassoux N Giron A Bodaghi B Tran TH Baudet S Davy F Chan CC Lehoang P & Merle-Béral H IL-10 measurement in aqueous humor for screening patients with suspicion of primary intraocular lymphoma. Invest Ophthalmol Vis Sci. 48(7) 3253 - 3259 2007. PMID: 17591896. DOI: 10.1167/iovs.06-0031
14 Sugita S Takase H Sugamoto Y Arai A Miura O & Mochizuki M Diagnosis of intraocular lymphoma by polymerase chain reaction analysis and cytokine profiling of the vitreous fluid. Jpn J Ophthalmol. 53(3) 209 - 214 2009. PMID: 19484437. DOI: 10.1007/s10384-009-0662-y
15 Coupland SE Heimann H & Bechrakis NE Primary intraocular lymphoma: A review of the clinical, histopathological and molecular biological features. Graefes Arch Clin Exp Ophthalmol. 242(11) 901 - 913 2004. PMID: 15565454. DOI: 10.1007/s00417-004-0973-0
16 Cheah CY Milgrom S Chihara D Gombos DS Pinnix CC Dabaja BS & Fowler NH Intensive chemoimmunotherapy and bilateral globe irradiation as initial therapy for primary intraocular lymphoma. Neuro Oncol. 18(4) 575 - 581 2016. PMID: 26487691. DOI: 10.1093/neuonc/nov253
17 Kaburaki T Taoka K Matsuda J Yamashita H Matsuda I Tsuji H Tanaka R Nakazaki K Nakamura F Kamiya K Kurokawa M Ohtomo K & Aihara M Combined intravitreal methotrexate and immunochemotherapy followed by reduced-dose whole-brain radiotherapy for newly diagnosed B-cell primary intraocular lymphoma. Br J Haematol. 179(2) 246 - 255 2017. PMID: 28699673. DOI: 10.1111/bjh.14848
18 Akiyama H Takase H Kubo F Miki T Yamamoto M Tomita M Mochizuki M Miura O & Arai A High-dose methotrexate following intravitreal methotrexate administration in preventing central nervous system involvement of primary intraocular lymphoma. Cancer Sci. 107(10) 1458 - 1464 2016. PMID: 27412324. DOI: 10.1111/cas.13012
19 Klimova A Heissigerova J Rihova E Brichova M Pytlik R Spicka I Mrazova K Karolova J & Svozilkova P Combined treatment of primary vitreoretinal lymphomas significantly prolongs the time to first relapse. Br J Ophthalmol. 102(11) 1579 - 1585 2018. PMID: 29378728. DOI: 10.1136/bjophthalmol-2017-311574
20 Fend F Ferreri AJ & Coupland SE How we diagnose and treat vitreoretinal lymphoma. Br J Haematol. 173(5) 680 - 692 2016. PMID: 27133587. DOI: 10.1111/bjh.14025
21 Smith JR Pe’er J Belfort RN Cardoso F Carvajal RD Carvalho C Coupland SE Desjardins L Francis JH Gallie BL Gombos DS Grossniklaus HE Heegaard S Jager MJ Kaliki S Ksander BR Maeurer M Moreno E Pulido JS Ryll B Singh AD Zhao J Parreira A Wilson DJ & O’Brien JM Proceedings of the association for research in vision and ophthalmology and champalimaud foundation ocular oncogenesis and oncology conference. Transl Vis Sci Technol. 8(1) 9 2018. PMID: 30652059. DOI: 10.1167/tvst.8.1.9
22 Bonzheim I Giese S Deuter C Süsskind D Zierhut M Waizel M Szurman P Federmann B Schmidt J Quintanilla-Martinez L Coupland SE Bartz-Schmidt KU & Fend F High frequency of MYD88 mutations in vitreoretinal B-cell lymphoma: A valuable tool to improve diagnostic yield of vitreous aspirates. Blood. 126(1) 76 - 79 2015. PMID: 25900979. DOI: 10.1182/blood-2015-01-620518
23 Miserocchi E Ferreri AJM Giuffrè C Cangi MG Francaviglia I Calimeri T Ponzoni M Pecciarini L Bandello FM & Modorati GM MYD88 L265P mutation detection in the aqueous humor of patients with vitreoretinal lymphoma. Retina. 39(4) 679 - 684 2019. PMID: 30204732. DOI: 10.1097/IAE.0000000000002319